Establishment of in vitro culture, plant regeneration and genetic transformation of Camelina sativa

Yemets A.I., Boychuk Yu.N., Shysha E.N., Rakhmetov D.B., Blume Ya.B.

SUMMARY. The results on in vitro culture establishment, plantlet regeneration and rooting of Camelina sativa cultivar sample Peremozhets and cultivar Mirazh are presented. Effective concentrations of sterilizing agents and duration of plant material treatment were estimated. Phytohormone ratio, sucrose concentration in nutrient medium that induce effective formation of C. sativa shoots and NAA concentration for plantlet rooting have been established. The method of Agrobacterium-mediated transformation of Camelina by using binary vector pGH217 carrying reporter β-glucoronidase (gus) gene driven under 35S CaMV promoter and nos-terminator, and selective marker hpt gene conferring hygromycin-resistance in transgenic plant was elaborated.

Tsitologiya i Genetika 2013, vol. 47, no. 3, pp. 14-20

E-mail: yemets.alla gmail.com

Yemets A.I., Boychuk Yu.N., Shysha E.N., Rakhmetov D.B., Blume Ya.B. Establishment of in vitro culture, plant regeneration and genetic transformation of Camelina sativa, Tsitol Genet., 2013, vol. 47, no. 3, pp. 14-20.

In "Cytology and Genetics":
A. I. Yemets, Yu. N. Boychuk, E. N. Shysha, D. B. Rakhmetov, Ya. B. Blume Establishment of in vitro culture, plant regeneration, and genetic transformation of Camelina sativa, Cytol Genet., 2013, vol. 47, no. 3, pp. 138-144
DOI: 10.3103/S0095452713030031