Corn plant DNA methylation pattern changes at fractionating of UV­C irradiation

Kravets A.P., Sokolova D.A., Vengjen G.S., Grodzinskiy D.M.

SUMMARY. Relationship of changes of methylation pattern of functionally different parts of DNA and chromosomal aberration yield was studied at the conditions of the fractionating of UV-С irradiation. Combination of restriction analysis (HpaII, MspI, MboI enzymes) with the subsequent raising of PCR ( internal transcribed space ITS1, ITS4 and inter simple sequence repeat – ISSR, 14b primers) was used. The got results testify to the changes in methylation pattern of satellite and transcription active part of DNA at an irradiation in the mode of fractionating and depending on fraction time ranges. The role of the methylation DNA pattern change in development of radiation damage and induction of organism protective reactions was discussed.

Key words: pattern of DNA methylation, transcribed DNA, sattelite DNA

Tsitologiya i Genetika 2013, vol. 47, no. 1, pp. 37-43

E-mail: kaplibra gmail.com

Kravets A.P., Sokolova D.A., Vengjen G.S., Grodzinskiy D.M. Corn plant DNA methylation pattern changes at fractionating of UV­C irradiation, Tsitol Genet., 2013, vol. 47, no. 1, pp. 37-43.

In "Cytology and Genetics":
A. P. Kravets, D. A. Sokolova, G. S. Vengzhen, D. M. Grodzinsky Corn plant DNA methylation pattern changes at UV- C irradiation fractionating, Cytol Genet., 2013, vol. 47, no. 1, pp. 29-33
DOI: 10.3103/S0095452713010052