TSitologiya i Genetika 2013, vol. 47, no. 1, 3-10
Cytology and Genetics 2013, vol. 47, no. 1, 1-7, doi: https://www.doi.org/10.3103/S0095452713010064

Thinopyrum 7Ai-1-derived small chromatin with Barley Yellow Dwarf Virus (BYDV) resistance gene integrated into the wheat genome with retrotransposon

Ma Y.-Z., Tomita M.

  • Molecular Genetics Laboratory, Faculty of Agriculture, Japan
  • Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing, China

Thinopyrum intermedium is a useful source of resistance genes for Barley Yellow Dwarf Virus (BYDV), one of the most damaging wheat diseases. In this study, wheat/Th. intermedium translocation lines with a BYDV resistance gene were developed using the Th. intermedium 7Ai-1 chromosome. Genomic in situ hybridization (GISH), using a Th. intermedium total genomic DNA probe, enabled detection of 7Ai-1-derived small chromatins containing a BYDV resistance gene, which were translocated onto the end of wheat chromosomes in the lines Y95011 and Y960843. Random amplified polymorphic DNA (RAPD) analyses using 120 random 10-mer primers were conducted to compare the BYDV-resistant translocation lines with susceptible lines. Two primers amplified the DNA fragments specific to the resistant line that would be useful as molecular markers to identify 7Ai-1-derived BYDV resistance chromatin in the wheat genome. Additionally, the isolated Th. intermedium-specific retrotransposon-like sequence pTi28 can be used to identify Th. intermedium chromatin transferred to the wheat genome.

Keywords: Thinopyrum intermedium, wheat, BYDV, 7Ai-1, GISH, RAPD, RFLP, retrotransposon

TSitologiya i Genetika
2013, vol. 47, no. 1, 3-10

Current Issue
Cytology and Genetics
2013, vol. 47, no. 1, 1-7,
doi: 10.3103/S0095452713010064

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