Combined influence of teichoic acids from Staphylococcus aureus and heterometallik Cu/Сd ethylenediamine complex on peritoneal macrophages and tumor cells

Nikulina V.V., Garmanchuk L.V., Senchylo N.V., Nikolaenko T.V., Dzhus O.I., Ostapchenko L.I., Khranovska N.M.

We investigated the effects of teichoic acid (TA) from Staphylococcus aureus Wood 46 on tumor growth and metastasis of the experimental Lewis lung carcinoma (LLC) in mice. Intranasal administration of TA alone aggravated both tumor growth and metastasis, whereas combined ad-ministration of TA with a synthetic bimetallic (copper : cadmium) ethylene diamine complex PO244 resulted in pronounced antitumor and antimetastatic effects. The group of animals subjected to the combined treatment with TA and PO244 manifested the highest degree of lymphocyte infiltration into the tumor tissue, compared to the control group and those exposed to TA or PO244 alone. Moreover, the combined treatment negatively affected the adhesive properties of peritoneal macrophages in the LLC bearing mice. Co-cultivation of the isolated macrophages with pri-mary LLC cultures revealed significant (p < 0.05) cytotoxic and cytostatic effects, detected as an increased level of apoptosis and a reduced fraction of replicating cells.

Key words: teichoic acids, Lewis lung carcinoma, bimetallic complex, macrophages

Tsitologiya i Genetika 2014, vol. 48, no. 6, pp. 56-61

• Educational and scientific centre «Institute of biology» of Taras Shevchenko National University of Kyiv
• National Cancer Institute, Kyiv

E-mail: liudmylagarmanchuk rambler.ru

Nikulina V.V., Garmanchuk L.V., Senchylo N.V., Nikolaenko T.V., Dzhus O.I., Ostapchenko L.I., Khranovska N.M. Combined influence of teichoic acids from Staphylococcus aureus and heterometallik Cu/Сd ethylenediamine complex on peritoneal macrophages and tumor cells, Tsitol Genet., 2014, vol. 48, no. 6, pp. 56-61.