TSitologiya i Genetika 2015, vol. 49, no. 2, 16-20
Cytology and Genetics , vol. , no. , , doi: https://www.doi.org/

Hydrogel nanoparticle encapsulated plasmid as a suitable gene delivery system

Rafiee A., Riazirad F., Alimohammadian M.H., T. Gazori, Fatemi S.M.R., Havaskary M.

  • Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
  • Immunology Department, Pasteur institute of Iran, Tehran, Iran
  • Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran
  • Islamic Azad University, Science and Research Branch, Department of Marine Biology, Tehran, Iran
  • Young researchers club, Tehran central branch, Islamic Azad University, Tehran, Iran

To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Na-noparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmid-complex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nano-particles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be Alg/Chi nanoparticles.

Keywords: chitosan, alginate, nanoparticle, size, transfection efficiency, cytotoxicity

TSitologiya i Genetika
2015, vol. 49, no. 2, 16-20

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