TSitologiya i Genetika 2018, vol. 52, no. 2, 39-50
Cytology and Genetics 2018, vol. 52, no. 2, 117–126, doi: https://www.doi.org/10.3103/S0095452718020032

Effects of exogenous phytohormones on spore germination and morphogenesis of Polystichum aculeatum (L.) roth gametophyte in culture in vitro

Babenko L.M., Romanenko K.O., Shcherbatiuk M.M., Vasheka O.V., Romanenko P.O.&Nbsp;, Negretsky V.A., Kosakivska I.V.

SUMMARY. The effects of exogenous phytohormones – gibberellic acid (GA) and benzyl-aminopurine (BAP) – on the spore germination and gametophyte morphogenesis of Polystichum aculeatum (L.) Roth in culture in vitro were studied. In the control, four stages of gametophyte morphogenesis were determined and their time periods were established. Spore germination and protonema formation of P. aculeatum occurred according to Vit-taria-type, prothalium development – according to Aspidium-type. The spores germination percentage depended on the storage time duration. It was found that pre-storage spore germination was 80–95 % in culture in vitro. Spores viability was within the range of 68 to 85 % after 4–6 month storage under lab conditions and did not exceed 20 % after 1.5 year of the storage period. High concentrations of exogenous GA3 (10–5
and 10–6 М) and BAP (10–5 М) were revealed to inhibit spore germination significantly whereas low concentrations (GA3 10–7–10–8 М) had an insignificant stimulating effect or did not affect germination at all (BAP 10–6, 10–7, 10–8 М). All concentrations of exoge-nous BAP were demonstrated to inhibit the develop-ment of P. aculeatum gametophyte at the protonema stage that might due to the apical dominance removing. The power of an inhibiting effect directly depended on BAP concentrations. The formation of abnormal thalli of P. aculeatum gametophyte in the response to exogenous GA3 treatments was observed. That abnormal form caused by damage of cell growth by elongation. A direct interrelationship between GA3 concentrations
and level of morphological abnormalities and grade of thalli underdevelopment was demonstrated.

Keywords: Polystichum aculeatum, gametophyte, protonema, prothallus, thallus, benzylaminopurine, BAP, gibberellic acid, GA3.

TSitologiya i Genetika
2018, vol. 52, no. 2, 39-50

Current Issue
Cytology and Genetics
2018, vol. 52, no. 2, 117–126,
doi: 10.3103/S0095452718020032

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