TSitologiya i Genetika 2018, vol. 52, no. 2, 91-93
Cytology and Genetics 2018, vol. 52, no. 2, 161–167, doi: https://www.doi.org/10.3103/S009545271802010X

A colon cancer related gene­MLH1, significantly affecting the pig litter size

Liu Yonggang, Chen Hongyu

  • Yunnan Key Laboratory of Fertility Regulation and Minority Birth Health , Kunming 650021, China
  • National Health and Family Planning Key Laboratory of Preconception Health in Western China,Kunming 650021, China
  • Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming 650201 China
  • Suizhou Hospital, Hubei University of Medicine, Suizhou 441300, China

MutL homolog 1, colon cancer, nonpolyposis type 2 (MLH1) is a tumor related gene. In this study, the full­length cDNA sequence of porcine MLH1 gene was cloned through rapid amplification of cDNA ends (RACE) method. The porcine MLH1 gene encodes a protein of 757 amino acids which shares high homology with the MLH1 of five species: chiru (96 %), sheep (95 %), cattle (93 %), southern­white­rhinoceros(92 %) and goat (92 %). This novel porcine gene was assigned to GeneID:100337665. Phylogenetic analysis revealed that the porcineMLH1 gene has a closer genetic relationship with theMLH1 gene of southern­white­rhinoceros. PCR­HhaI­RFLP was established to detect GU373696:c.395 G > A substitution of porcine MLH1 gene and eight pig breeds display obvious genotype and allele frequency differences at this mutation locus. Association of this SNP with litter size traits was assessed in Large White (n = 200) and Landrace (n = 200) pig populations, and result demonstrated that this polymorphic locus was significantly associated with the litter size of all parities in Large White and Landrace sows (P < 0.01). Therefore, MLH1 is also a reproduction related gene.

Keywords: pig; MLH1; polymorphism; litter size; re­production

TSitologiya i Genetika
2018, vol. 52, no. 2, 91-93

Current Issue
Cytology and Genetics
2018, vol. 52, no. 2, 161–167,
doi: 10.3103/S009545271802010X

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