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Zygotic autopoliploidization of rye (Secale cereale L.)
SUMMARY. The paper presents the results of zygotic autopoly-ploidization by the nitrous oxide (N2O) of diploid cultivars and F1 hybrids of winter rye. It has been shown that the method of creating zygotic rye tetraploids by the nitrous oxide (N2O) is effective and allows obtaining up to 86,0 % of tetraploids. The average yield of tetraploids was 51,0 %. By this method we created 9 new rye tetraploids. The created tetraploids are characterized by chromosomal balance and low levels of aneuploidy (up to 9,7 %). Meiosis in rye zygotic tetraploids occurs with significantly more defections (P ≤ 0,05) than in the original diploids. It has been established that in the created tetraploids, part of the nuclear DNA is eliminated to the C6–C7 generations. On the basis of the obtained tetraploid Yubileinaya, a new variety of winter tetraploid rye Camea 16 was created.
Key words: winter rye, nitrous oxide, diploids, zygotic tetraploids, meiosis, DNA elimination
E-mail: I_Gordej777 mail.ru
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