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Effect of platelet rich plasma on morphogenesis and chondrogenic marker gene expression by chondrocyte-like rat Nucleus pulposus cells in vitro

Pedachenko E.G., Vasilyeva I.G., Khizniak M.V., Chopyck N.G., Oleksenko N.P., Shuba I.N., Tsjubko O.I., Galanta O.S., Dmytrenko A.B., Makarova T.A., Snitsar N.D.


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SUMMARY. The purpose was to study the effect of 3, 10 and 20 % rat platelet rich plasma (PRP) on the morphobiologic characteristics of chondrocyte-like rat nucleus pulposus (NP) cells, and also expression of chondrogenic genes: ACAN, COL II, GPC3, MGP, PTN, ANXA3; VIM in vitro. The viability of rat NP cells in culture is dose-dependently enhanced by 3, 10, 20 % PRP. Platelets in cultures show tropicity to blast-like cells, form an adhesion zone. When 20 % PRP are used at the 14th day of cultivation, the blast-like cells are differentiated, the cells are recorded at the stage of division. The decreased expression of chondrogenic genes is dose-dependently prevented by PRP. More effective was 20 % PRP. In these cultures, an increase in the expression of most studied chondrogenic genes was observed: 2,9 times higher for COL II (P = 0,002); 2,1 times higher for VIM (P = 0.0003); 2,3 times higher for ANXA (P = 0,0003); 1,4 times higher for GPC (P = 0.0011); 1,6 times higher for PTN (P = 0,0017), 1,2 times higher for ACAN (P = 0,0606).

Tsitologiya i Genetika 2019, vol. 53, no. 2, pp. 34-42

E-mail: pedach, vigvasileva, Khyzhnyak63, natalia.chopyck, n.oleksenko, irina_shuba, tsjubkoolga, lenagalanta, 1866600, tatyana.makarova77799, angelikdmiz.bor

Pedachenko E.G., Vasilyeva I.G., Khizniak M.V., Chopyck N.G., Oleksenko N.P., Shuba I.N., Tsjubko O.I., Galanta O.S., Dmytrenko A.B., Makarova T.A., Snitsar N.D. Effect of platelet rich plasma on morphogenesis and chondrogenic marker gene expression by chondrocyte-like rat Nucleus pulposus cells in vitro, Tsitol Genet., 2019, vol. 53, no. 2, pp. 34-42.

In "Cytology and Genetics":
E. G. Pedachenko, I. G. Vasilyeva, M. V. Khizniak, N. G. Chopyck, N. P. Oleksenko, I. N. Shuba, O. I. Tsjubko, O. S. Galanta, N. D. Snitsar, T. A. Makarova, A. B. Dmytrenko The Effect of Platelet-Rich Plasma on Morphogenesis and Expression of Chondrogenic Marker Genes by Chondrocyte-Like Cells of the Rat Nucleus Pulposus in Vitro, Cytol Genet., 2019, vol. 53, no. 2, pp. 124–131
DOI: 10.3103/S0095452719020087


1. Daly, C., Ghosh, P., Jenkin, G., Oehme, D., and Goldschlager, T., A Review of Animal Models of Intervertebral Disc Degeneration: Pathophysiology, Regeneration, and Translation to the Clinic, Biomed. Res. Int., 2016, vol. 2016. 5952165.

2. Anitua, E. and Padilla, S., Biologic therapies to enhance intervertebral disc repair, Regen. Med., 2018, vol. 13, no. 1, pp. 55–72.

3. Fernandes, G. and Yang, S., Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering, Bone Res., 2016, vol. 4, p. 16036.

4. Wang, S., Fan, W., Jia, J., Ma, L., Yu, J., and Wang, C., Is exclusion of leukocytes from platelet-rich plasma (PRP) a better choice for early intervertebral disc regeneration?, Stem Cell Res. Ther., 2018, vol. 9, p. 199.

5. Oloff, L., Elmi, E., Nelson, J., and Crain, J., Retrospective analysis of the effectiveness of platelet-rich plasma in the treatment of achilles tendinopathy: pretreatment and posttreatment correlation of magnetic resonance imaging and clinical assessment, Foot Ankle Spec., 2015, vol. 8, no. 6, pp. 490–497.

6. Gosens, T., Peerbooms, J.C., van Laar, W., and Oudsten, B.L., Ongoing positive effect of platelet-rich plasma versus corticosteroid injection in lateral epicondylitis: a double-blind randomized controlled trial with 2-year follow-up, Am. J. Sports Med., 2011, vol. 39, no. 6, pp. 1200–1208.

7. Laver, L., Marom, N., Dnyanesh, L., Mei-Dan, O., Espregueira-Mendes, J., and Gobbi, A., PRP for degenerative cartilage disease: a systematic review of clinical studies, Cartilage, 2017, vol. 8, no. 4, pp. 341–364.

8. Kon, E., Buda, R., Filardo, G., Di Martino, A., Timoncini, A., Cenacchi, A., Fornasari, P.M., Giannini, S., and Marcacci, M., Platelet-rich plasma intraarticular knee injections produced favorable results on degenerative cartilage lesions, Knee Surg Sports Traumatol Arthrosc., 2010, vol. 18, no. 4, pp. 472–479.

9. Kon, E., Filardo, G., Delcogliano, M., Presti, M.L., Russo, A., Bondi, A., Di Martino, A., Cenacchi, A., Fornasari, P.M., and Marcacci, M., Platelet-rich plasma: New clinical application: a pilot study for treatment of jumper’s knee, Injury, 2009, vol. 40, no. 6, pp. 598–603.

10. Vannini, F., Di Matteo, B., and Filardo, G., Platelet-rich plasma to treat ankle cartilage pathology - from translational potential to clinical evidence: a systematic review, J. Exp. Orthopaedics, 2015, vol. 2, p. 2.

11. Louis, M., Magalon, J., Jouve, E., Bonet, C.E., Mattei, J.C., Chagnaud, C., Rochewerger, A., Veran, J., and Sabatier, F., Growth factors levels determine efficacy of platelets rich plasma injection in knee osteoarthritis: a randomized double blind noninferiority trial compared with viscosupplementation arthroscopy, J. Arthrosc. Rel. Surgery, 2018, vol. 34, no. 5, pp. 1530–1540.

12. Macaulay, I.C., Carr, P., Gusnanto, A., Ouwehand, W.H., Fitzgerald, D., and Watkins, N.A., Platelet genomics and proteomics in human health and disease, J. Clin. Invest., 2005, vol. 115, no. 12, pp. 3370–3377.

13. Garcia, A., Platelet clinical proteomics: facts, challenges, and future perspectives, Proteomics Clin. Appl., 2016, vol. 10, no. 8, pp. 767–773.

14. Fortier, L.A., Barker, J.U., Strauss, E.J., McCarrel, T.M., and Cole, B.J., The role of growth factors in cartilage repair, Clin. Orthop. Rel. Res., 2011, vol. 469, no. 10, pp. 2706–2715.

15. Dolder, J., Mooren, R., Vloon, A., Stoelinga, P., and Jansen, J., Platelet-rich plasma: quantification of growth factor levels and the effect on growth and differentiation of rat bone marrow cells, Tissue Eng., 2006, vol. 12, no. 11, pp. 3067–3073.

16. Este, M., Bara, J.J., Denom, J., Menzel, U., Alini, M., Verrier, S., and Herrmann, M., An In Vitro investigation of platelet-rich plasma-gel as a cell and growth factor delivery vehicle for tissue engineering, Tiss. Eng. Part C, Methods, 2016, vol. 22, no. 1, pp. 49–58.

17. Fioravanti, C., Frustaci, I., Armellin, E., Condo, R., Arcuri, C., and Cerroni, L., Autologous blood preparations rich in platelets, fibrin and growth factors, Oral. Implant., 2015, vol. 8, no. 4, pp. 96–113.

18. Akeda, K., An, H., Okuma, M., Attawia, M., Miyamoto, K., Thonar, E.G., Lenz, M.E., Sah, R.L., and Masuda, K., Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis, Osteoarthr. Cartil., 2006, vol. 14, no. 12, pp. 1272–1280.

19. Spreafico, A., Chellini, F., Frediani, B., Bernardini, G., Niccolini, S., Serchi, T., Collodel, G., Paffetti, A., Fossombroni, V., Galeazzi, M., Marcolongo, R., and Santucci, A., Biochemical investigation of the effects of human platelet releasates on human articular chondrocytes, J. Cell Biochem., 2009, vol. 108, no. 5, pp. 1153–1165.

20. Hildner, F., Eder, M.J., Hofer, K., Aberl, J., Redl, H., van Griensven, M., Gabriel, C., and Peterbauer-Scherb, A., Human platelet lysate successfully promotes proliferation and subsequent chondrogenic differentiation of adipose-derived stem cells: a com parison with articular chondrocytes, Tiss. Eng. Regen. Med., 2015, vol. 9, no. 7, pp. 808–818.

21. Gaissmaier, C., Fritz, J., Krackhardt, T., Flesch, I., Aicher, W., and Ashammakhi, N., Effect of human platelet supernatant on proliferation and matrix synthesis of human articular chondrocytes in monolayer and three-dimensional alginate cultures, Biomaterials, 2005, vol. 26, no. 14, pp. 1953–1960.

22. Muraglia, A., Nguyen, V.T., Nardini, M., Mogni, M., Coviello, D., Dozin, B., Strada, P., Baldelli, I., Formica, M., Cancedda, R., and Mastrogiacomo, M., Culture medium supplements derived from human platelet and plasma: cell commitment and proliferation support, Front. Bioeng. Biotechnol., 2017, vol. 5, p. 66.

23. Chien, C., Ho, H., Liang, Y., Ko, P., Sheu, M., and Chen, C., Incorporation of exudates of human platelet-rich fibrin gel in biodegradable fibrin scaffolds for tissue engineering of cartilage, J. Biomed. Mater. Res. Part B:, Appl. Biomater., 2012, vol. 100, no. 4, pp. 948–955.

24. Zhou, Z., Yu, H., Wang, Y., Guo, Q., Wang, L., and Zhang, H., Z, ZNF606 interacts with Sox9 to regulate chondrocyte differentiation, Biochem. Biophys. Res. Commun., 2016, vol. 479, no. 4, pp. 920–926.

25. Xu, C., Zhu, S., Wu, M., Han, W., and Yu, Y., Functional receptors and intracellular signal pathways of midkine (MK) and pleiotrophin (PTN), Biol. Pharmac. Bull., 2014, vol. 37, no. 4, pp. 511–520.

26. Vo, N.V., Hartman, R.A., Patil, P.R., Risbud, M.V., Kletsas, D., Iatridis, J.C., Hoyland, J.A., Le Maitre, C.L., Sowa, G.A., and Kang, J.D., Molecular mechanisms of biological aging in intervertebral discs, J. Orthop. Res., 2016, vol. 34, no. 8, pp. 1289–1306.

27. Ngo, K., Patil, P., McGowan, S.J., Niedernhofer, L.J., Robbins, P.D., Kang, J., Sowa, G., and Vo, N., Senescent intervertebral disc cells exhibit perturbed matrix homeostasis phenotype, Mech. Ageing Dev, 2017, vol. 166, pp. 16–23.

28. Ngo, K., Patil, P., McGowan, S.J., Niedernhofer, L.J., Robbins, P.D., Kang, J., Sowa, G., and Vo, N., Current strategies for treatment of intervertebral disc degeneration: substitution and regeneration possibilities, Biomater. Res., 2017, vol. 21, p. 22.

29. Vynios, D.H., Metabolism of cartilage proteoglycans in health and disease, Biomed. Res. Int., 2014, vol. 2014. 452315.

30. Neill, T., Schaefer, L., and Iozzo, R.V., Decoding the matrix: Instructive roles of proteoglycan receptors, Biochemistry, 2015, vol. 54, no. 30, pp. 4583–4598.

31. Chen, S., Fu, P., Wu, H., and Pei, M., Meniscus, articular cartilage, and nucleus pulposus: a comparative review of cartilage-like tissues in anatomy, development, and function, Cell Tiss. Res., 2017, vol. 370, no. 1, pp. 53–70.

32. Rodrigues-Pinto, R., Richardson, S., and Hoyland, J., Identification of novel nucleus pulposus markers, Bone Joint Res., 2013, vol. 2, no. 8, pp. 169–178.

33. Tapp, H., Hernandez, D., Neame, P., and Koob, T., Pleiotrophin inhibits chondrocyte proliferation and stimulates proteoglycan synthesis in mature bovine cartilage, Matrix Biol., 1999, vol. 18, no. 6, pp. 543–556.

34. Lee, C., Sakai, D., Nakai, T., Toyama, K., Mochida, J., Alini, M., and Grad, S., A phenotypic comparison of intervertebral disc and articular cartilage cells in the rat, Eur. Spine J., 2007, vol. 16, no. 12, pp. 2174–2185.

35. Schloer, S., Pajonczyk, D., and Rescher, U., Annexins in translational research: hidden treasures to be found, Int. J. Mol. Sci., 2018, vol. 19, no. 6, p. 1781.

36. Luo, G., Ducy, P., McKee, M., Pinero, G.J., Loyer, E., Behringer, R.R., and Karsenty, G., Spontaneous calcification of arteries and cartilage in mice lacking matrix GLA protein, Nature, 1997, vol. 386, no. 6620, pp. 78–81.

37. Illien-Jünger, S., Torre, O.M., Kindschuh, W.F., Chen, X., Laudier, D.M., and Iatridis, J.C., Ages induce ectopic endochondral ossification in intervertebral discs, Eur. Cell Mater., 2016, vol. 18, no. 32, pp. 257–270.

38. Fontes, R.B., Baptista, J.S., Rabbani, S.R., Traynelis, V.C., and Liberti, E.A., Structural and ultrastructural analysis of the cervical discs of young and elderly humans, PLoS One, 2015, vol, 10, no. 10. e0139283.

39. Newman, B., Gigout, L., Sudre, L., Grant, M., and Wallis, G., Coordinated expression of matrix Gla protein is required during endochondral ossification for chondrocyte survival, J. Cell Biol., 2001, vol. 154, no. 3, pp. 659–666.

40. Lv, F., Leung, V.Y., Huang, S., Huang, Y., Sun, Y., and Cheung, K.M., In search of nucleus pulposus-specific molecular markers, Rheumatology (Oxford), 2014, vol. 53, no. 4, pp. 600–610.

41. Lee, H., Shon, O., Park, S., Kim, H.J., Kim, S., Ahn, M.W., and Do, S.H., Platelet-rich plasma increases the levels of catabolic molecules and cellular dedifferentiation in the meniscus of a rabbit model, Int. J. Mol. Sci., 2016, vol. 17, no. 1, p. 120.

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