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Expression profiles of a tung tree phosphate transporter cdna and structural characteristics of the encoded protein
Plant phosphate transporters mediate phosphate acquisition, translocation, and recycling. A Vernicia fordii cDNA encoding a phosphate transporter was cloned using a reverse transcription polymerase chain reaction (PCR) and rapid amplification of cDNA ends. The resulting cDNA named Vernicia fordii Phosphate Transporter1;1 (VfPht1;1) encodes a predicted protein consisting of 533 amino acids. This protein, which was predicted to contain 12 transmembrane domains, is most similar (89,9 %) to a Ricinus communis predicted protein. The quantitative realtime PCR data revealed that VfPht1;1 is most highly expressed in mature leaf petioles. It is also highly expressed in seeds collected in June, when developing fruits require a large amount of phosphates. Our observations suggest that VfPht1;1 may have diverse functions related to phosphate uptake and translocation.
Key words: Vernicia fordii; phosphate transporter; VfPT1;1; gene cloning; gene expression
The Key Lab of Nonwood Forest Nurturing and Protection of the National Ministry of Education, Central South University of Forestry and Technology, Changsha 410004
E-mail: tanxiaofengcn 126.com, zhoujunqin1234 126.com; yuanjunchina 126.com; longhx886 126.com
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