TSitologiya i Genetika 2019, vol. 53, no. 1, 78-80
Cytology and Genetics 2019, vol. 53, no. 1, 86–95, doi: https://www.doi.org/10.3103/S0095452719010031

Isolation, characterization and association among phosphate solubilizing bacteria from sugarcane rhizosphere

M. Awais, M. Tariq, Q. Ali, A. Khan, A. Ali, I. A. Nasir, T. Husnain

  • Centre of Excellence in Molecular Biology, University of the Punjab, Lahore-53700, Pakistan
  • Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan

One of the premium qualities of Phosphate solubilizing bacteria is to solubilize insoluble phosphorus to make it available for plant roots to be engrossed. To check the ability for phosphate solubilization, production of indole acetic acid, antagonistic activity against fungal pathogen and intrinsic antibiotic resistance phosphorous solubilizing bacterial isolates were isolated and screened. In total, 12 PSB were found rod shaped cells being gram negative.  Different levels of antibiotic resistance were observed by rhizobacterial isolates against four antibiotics (Ampicillin, Kanamycin, Tetracycline and Streptomycin 25, 30, 30 and 10 mg/ml respectively). The isolates S7 and S20 showed antifungal activity against Fusarium oxysporum. Conversion of insoluble phosphorous Ca3 (PO4) into IAA was observed by all PSB Isolates. Two phosphorous solubilizing bacterial isolates sequences were submitted in NCBI database. Conclusively, good antifungal activity with greater ability to solubilize insoluble phosphorus can be achieved by combine application of rhizobacterial isolates with S22. Further, it is an eco-friendly and cost-effective strategy to improve crop production.

Keywords: phosphate solubilizing bacteria, 16S rRNA, antagonistic activity, Fusarium oxysporum, rhizobacteria

TSitologiya i Genetika
2019, vol. 53, no. 1, 78-80

Current Issue
Cytology and Genetics
2019, vol. 53, no. 1, 86–95,
doi: 10.3103/S0095452719010031

Full text and supplemented materials

References

1. Kumar, A., Bhargava, P., and Rai, L.C., Isolation and molecular characterization of phosphate solubilizing Enterobacter and Exiguobacterium species from paddy fields of Eastern Uttar Pradesh, India, Afric. J. Microbiol. Res., 2010, vol. 4, pp. 820–829.

2. Mahidi, S., Hassan, G., and Hussain, A., and Faisal-ur-Rasool, Phosphorus availability issue. Its fixation and role of phosphate solubilizing bacteria in phosphate solubilization case study, Res. J. Agric. Sci., 2011, vol. 2, pp. 2174–179.

3. Chen, Y., Rekha, P., Arun, A., Shen, F., Lai, W.A., et al., Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities, Appl. Soil Ecol., 2006, vol. 34, pp. 33–41.

4. Vessey, J.K., Plant growth promoting rhizobacteria as biofertilizers, Plant Soil, 2003, vol. 255, pp. 571–586.

5. Kiani, S., Ali, A., Bajwa, K.S., Muzaffar, A., Ashraf, M.A., and Husnain, T., Cloning and chloroplast-targeted expression studies of insect-resistant gene with ricin fusion-gene under chloroplast transit peptide in cotton, Electronic J. Biotechnol., 2013, vol. 16, pp. 13–13.

6. Wakelin, S.A., Warren, R.A., Harvey, P.R., and Ryder, M.H., Phosphate solubilization by Penicillium spp. closely associated with wheat roots, Biol. Fert. Soil, 2004, vol. 40, pp. 36–43.

7. Zaidi, A., Khan, M., Ahemad, M., and Oves, M., Plant growth promotion by phosphate solubilizing bacteria, Acta Microbiol. Immunol. Hungar., 2009, vol. 56, pp. 263–284.

8. Song, O.R., Lee, S.J., Lee, Y.S., Lee, S.C., and Kim, K.K., Solubilization of insoluble inorganic phosphate by Burkholderia cepacia DA23 isolated from cultivated soil, Brazil. J. Microbiol., 2008, vol. 39, pp. 151–156.

9. Ahmed, N. and Shahab, S., Phosphate solubilization: their mechanism, genetics and application, Internet J. Microbiol., 2011, vol. 9, pp. 4408–4412.

10. Dhandapani, P., Insoluble phosphate solubilization by bacterial strains isolated from rice rhizosphere soils from Southern India, Int. J. Soil Sci., 2011, vol. 6, pp. 134–141.

11. Tariq, M., Ali, Q., Khan, A., Khan, G.A., Rashid, B., and Husnain, T., Yield potential study of Capsicum annuum L. under the application of PGPR, Advan. Life Sci., 2014, vol. 1, pp. 202–207.

12. Richardson, A.E., Hadobas, P.A., Hayes, J.E., O’hara, C., and Simpson, R., Utilization of phosphorus by pasture plants supplied with myo-inositol hexaphosphate is enhanced by the presence of soil microorganisms, Plant Soil, 2001, vol. 229, pp. 47–56.

13. Dar, A.I., Saleem, F., Ahmad, M., Tariq, M., Khan, A., Ali, Q., Nasir, I.A., and Husnain, T., Characterization and efficiency assessment of PGPR for enhancement of rice (Oryza sativa L.) yield, Advan. Life Sci., 2014, vol. 2, pp. 38–45.

14. Ali, Q., Ali, A., Awan, M.F., Tariq, M., Ali, S., Nasir, I.A., and Husnain, T., Combining ability analysis for various physiological, grain yield and quality traits of Zea mays L., Life Sci. J., 2014, vol. 11, pp. 540–551.

15. Ali, Q., Ali, A., Ahsan, M., Nasir, I.A., Abbas, H.G., and Husnain, T., Line Tester analysis for morpho-physiological traits of Zea mays L. seedlings, Advan. Life Sci., 2014, vol. 1, pp. 242–253.

16. Ali, Q., Ahsan, M., Ali, F., Aslam, M., and Khan, N.H., Heritability, heterosis and heterobeltiosis studies for morphological traits of maize (Zea mays L.) seedlings, Advan. Life Sci., 2013, vol. 1, pp. 52–63.

17. Pikovskaya, R., Mobilization of phosphorus in soil in connection with vital activity of some microbial species, Mikrobiologiya, 1948, vol. 17, pp. 362–370.

18. Nautiyal, C.S., An efficient microbiological growth medium for screening phosphate solubilizing microorganisms, FEMS Microbiol. Lett., 1999, vol. 170, pp. 265–270.

19. Gen-Fu, W. and Xue-Ping, Z., Characterization of phosphorus-releasing bacteria in a small eutrophic shallow lake, Eastern China, Water Res., 2005, vol. 39, pp. 4623–4632.

20. DeFreitas, J., Banerjee, M., and Germida, J., Phosphate-solubilizing rhizobacteria enhance the growth and yield but not phosphorus uptake of canola (Brassica napus L.), Biol. Fert. Soil, 1997, vol. 24, pp. 358–364.

21. Perez, E., Sulbaran, M., Ball, M.M., and Yarzabal, L.A., Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region, Soil Biol. Biochem., 2007, vol. 39, pp. 2905–2914.

22. Panhwar, Q.A., Othman, R., Rahman, Z.A., Meon, S., and Ismail, M.R., Isolation and characterization of phosphate-solubilizing bacteria from aerobic rice, Afric. J. Biotechnol., 2014, vol. 11, pp. 2711–2719.

23. Illmer, P. and Schinner, F., Solubilization of inorganic calcium phosphates-solubilization mechanisms, Soil Biol. Biochem., 1995, vol. 27, pp. 257–263.

24. Yasmin, F., Othman, R., Sijam, K., and Saad, M.S., Characterization of beneficial properties of plant growth-promoting rhizobacteria isolated from sweet potato rhizosphere, Afric. J. Microbiol. Res., 2010, vol. 3, pp. 815–821.

25. Steel, R.T., James, H., and Dicky, A.D., Principles and Procedures of Statistics: A Biometrical Approach, Chapter: Book Name, McGraw-Hill, New York New York, USA, 1997, pp. 400–428.

26. Pradhan, N. and Sukla, L., Solubilization of inorganic phosphates by fungi isolated from agriculture soil, Afric. J. Biotechnol., 2006, vol. 5, pp. 850–854.

27. Mehta, S. and Nautiyal, C.S., An efficient method for qualitative screening of phosphate-solubilizing bacteria, Curr. Microbiol., 2001, vol. 43, pp. 51–56.

28. DeSouza, R., Beneduzi, A., Ambrosini, A., Costa, P.B., and Meyer, J., The effect of plant growth-promoting rhizobacteria on the growth of rice (Oryza sativa L.) cropped in southern Brazilian fields, Plant Soil, 2013, vol. 366, pp. 585–603.

29. Glick, B.R. and Pasternak, J.J., Principles and applications of recombinant DNA, Mol. Biotechnol., 1998, vol. 683.

30. Woo, S.M., Lee, M., Hong, I., Poonguzhali, S., and Sa, T., Isolation and characterization of phosphate solubilizing bacteria from Chinese cabbage, in 19th World Congress of Soil Science, Soil Solutions for a Changing World, 2010, pp. 1–6.

31. Noor, N.M., Kean, C.W., Vun, Y.L., and Mohamed-Hussein, Z.A., In vitro conservation of Malaysian biodiversity—achievements, challenges and future directions, In Vitro Cell. Dev. Biol.—Plant, 2011, vol. 47, pp. 26–36.

32. Afzal, M., Ali, M.I., Munir, M.A., Ahmad, M., Mahmood, Z., Sharif, M.N., and Aslam, M., Genetic association among morphological traits of Lepidium draba, Bull. Bio. All. Sci. Res., 2016, vol. 1, pp. 1–5.

33. Ahmad, M., Munir, M.A., Mahmood, Z., Ali, M.I., Afzal, M., Sharif, M.N., and Khan, T.M., Multivariate analysis for morphological traits of Convulvularis arvensis, Bull. Bio. All. Sci. Res., 2016, vol. 1, pp. 1–6.

34. Ali, M., Mahmood, Z., Ahmad, M., Afzal, M., Munir, M.A., Sharif, M.N., and Shakeel, A., Genetic variability in Cirsium arvensei under different environmental conditions, Bull. Bio. All. Sci. Res., 2016, vol. 1, pp. 1–4.

35. Mahmood, Z., Afzal, M., Ahmad, M., Munir, M.A., Ali, M.I., Sharif, M.N., and Maqbool, R., Genetic analysis for morphological traits of Euphorbia helioscopia, Bull. Bio. All. Sci. Res., 2016, vol. 1, pp. 1–4.

36. Munir, M., Ahmad, M., Ali, M.I., Mahmood, Z., Afzal, M., Sharif, M.N., and Aslam, M., Correlation and regression analysis of morphological traits in Rumex dentatus, Bull. Bio. All. Sci. Res., 2016, vol. 1, pp. 1–5.

37. Xiao, C., Chi, R., Li, X., Xia, M., and Xia, Z., Biosolubilization of rock phosphate by three stress-tolerant fungal strains, Appl. Biochem. Biotechnol., 2011, vol. 165, pp. 719–727.

38. Colwell, J., The estimation of the phosphorus fertilizer requirements of wheat in southern New South Wales by soil analysis, Anim. Prod. Sci., 1963, vol. 3, pp. 190–197.

39. Ali, Q., Ali, A., Awaan, M.F., Ahmed, S., Nazar, Z.A., Akram, F., Shahzad, A., Nasir, I.A., and Husnain, T., Gene action for various grain and fodder quality traits in Zea mays, J. Food Nutr. Res., 2014, vol. 2, pp. 704–717.