TSitologiya i Genetika 2019, vol. 53, no. 6, 79-82
Cytology and Genetics 2019, vol. 53, no. 6, 494–501, doi: https://www.doi.org/10.3103/S0095452719060057

Phenolic com-pounds of Codiaeum varie-gatum spirale lessened cytotoxic and genotoxic effects of mitomycin C in mice somatic and germ cells

MONA A.M. ABO-ZEID, AYMAN A. FARG-HALY, EMAD M. HASSAN, NEGM S. ABDEL-SAMIE

  • Genetics and Cytology Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki 12622, Cairo, Egypt
  • Medicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research division, National Research Centre, Dokki 12622, Cairo, Egypt

This study evaluates the chemopreventive properties of phenolic compounds extracted from Codiaeum variegatum (PCCV) against cytotoxic and genotoxic effects of mitomycin C (MMC). Male Swiss albino mice were treated with PCCV and/or MMC, and samples were collected 24 h after the last treatments. We recorded chromosome aberrations in bone-marrow cells and spermatocytes, cell viability and DNA damage (using comet assay) in bone-marrow cells. We observed that the highest concentration of PCCV did not induce cytotoxic or genotoxic effects on mice somatic and germ cells. However, MMC reduced cell proliferation remarkably (p < 0.05), and increased chromosome aberrations and DNA damage significantly. Additionally, PCCV prohibited cytotoxicity and genotoxicity of MMC when administered to animals 2 h prior to MMC. Evidently, PCCV induced a significant inhibition (p < 0.05) in the percentages of chromosome aberrations in bone-marrow cells excluding gaps (19.82%, 25.23 %, and 42.34 %) and spermatocytes (22.73 %, 31.82 %, and 48.48 %) at concentrations 125, 250 and 500 mg/kg b.wt respectively. Likewise, PCCV declined tail DNA (%), tail length (µm) and tail moment, and the inhibitory index of tail DNA (%) reached to 89.44 % at 500 mg/kg b.wt. In conclusion, phenolic compounds of Codiaeum variegatum reduced the cytotoxic and genotoxic effects that were induced by the mutagenic agent MMC.

Keywords: Codiaeum variegatum; Phenolic compounds; Cytotoxicity; Genotoxicity; Chromosome aberration; Comet assay

TSitologiya i Genetika
2019, vol. 53, no. 6, 79-82

Current Issue
Cytology and Genetics
2019, vol. 53, no. 6, 494–501,
doi: 10.3103/S0095452719060057

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