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Phenolic com-pounds of Codiaeum varie-gatum spirale lessened cytotoxic and genotoxic effects of mitomycin C in mice somatic and germ cells
This study evaluates the chemopreventive properties of phenolic compounds extracted from Codiaeum variegatum (PCCV) against cytotoxic and genotoxic effects of mitomycin C (MMC). Male Swiss albino mice were treated with PCCV and/or MMC, and samples were collected 24 h after the last treatments. We recorded chromosome aberrations in bone-marrow cells and spermatocytes, cell viability and DNA damage (using comet assay) in bone-marrow cells. We observed that the highest concentration of PCCV did not induce cytotoxic or genotoxic effects on mice somatic and germ cells. However, MMC reduced cell proliferation remarkably (p < 0.05), and increased chromosome aberrations and DNA damage significantly. Additionally, PCCV prohibited cytotoxicity and genotoxicity of MMC when administered to animals 2 h prior to MMC. Evidently, PCCV induced a significant inhibition (p < 0.05) in the percentages of chromosome aberrations in bone-marrow cells excluding gaps (19.82%, 25.23 %, and 42.34 %) and spermatocytes (22.73 %, 31.82 %, and 48.48 %) at concentrations 125, 250 and 500 mg/kg b.wt respectively. Likewise, PCCV declined tail DNA (%), tail length (µm) and tail moment, and the inhibitory index of tail DNA (%) reached to 89.44 % at 500 mg/kg b.wt. In conclusion, phenolic compounds of Codiaeum variegatum reduced the cytotoxic and genotoxic effects that were induced by the mutagenic agent MMC.
Key words: Codiaeum variegatum; Phenolic compounds; Cytotoxicity; Genotoxicity; Chromosome aberration; Comet assay
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