TSitologiya i Genetika 2020, vol. 54, no. 1, 33-41
Cytology and Genetics 2020, vol. 54, no. 1, 23–30, doi: https://www.doi.org/10.3103/S0095452720010089

Endogenous phytohormones of fern Polystichum aculeatum (L.) Roth gametophytes at different stages of morphogenesis in vitro culture

Kosakivska I.V., Vasyuk V.A., Voytenko L.V., Shcherbatiuk M.M., Romanenko K.O., Babenko L.M.

  • M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv, Ukraine

SUMMARY. The content of indolyl-3-acetic (IAA), gibberellic (GA3), abscisic acids (ABA) and cytokinins – zeatin (Z), zeatin-O-glucoside (ZG), zeatinriboside (ZP), isopentenyladenine (iP) and isopentenyladenosine (iPA) was determined using the method of high-performance liquid chromatography in fern Polystichum aculeatum gametophytes at different stages of its morphogenesis in in vitro culture. It was shown that at the stage of spatulate prothallium development the gametophytes contained significant amounts of GA3 and IAA, while the content of ABA and cytokinins was low. The inactive ZG and active iP dominated. At the stage of cordiform thallus formation the level of GA3 increased by 1.6, IAA – by 2.4, ABA – by 1.7 times. The content of inactive iPA increased twice, the level of active ZR and iP increased significantly. At the stage of sporophyte development on the surface of gametophyte thallus the levels of IAA, ABA and GA3 decreased. The accumulation of inactive ZG and decrease in the content of active zeatin and zeatinriboside were documented. At all stages of gametophytes morphogenesis GA3 was dominant, the highest content of it was detected during the development of archegonium and antheridium. The character of gibberellic acid and zeatinriboside accumulation has shown the key role of these hormones in the regulation of growth processes and the development of reproductive structures of P. aculeatum gametophytes.

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TSitologiya i Genetika
2020, vol. 54, no. 1, 33-41

Current Issue
Cytology and Genetics
2020, vol. 54, no. 1, 23–30,
doi: 10.3103/S0095452720010089

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