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Development of ukrainian breeding winter rape Brassica napus L. an effective in vitro regeneration system

Hnatiuk I.S., Varchenko O.I., Kuchuk M.V., Parii M.F., Symonenko Yu.V.

 




SUMMARY. The regeneration method of the commercial line Bn1 winter rape was optimized. Hypocotyls fragments of 6-day-old seedlings were used as explants. Regeneration occurred by organogenesis on a MS nutrient medium supplemented with 3 mg/L of benzylaminopurine and 2 mg/L of 2-isopentyladenine. All obtained regenerant plants were successfully rooted in a hormone-free nutrient medium and adapted to soil conditions. The selected vernalization conditions provided 83,93 5,33 % of budding and flowering. PCR analysis using ISSR 2 and ISSR 15 markers showed that somaclonal variation does not occur in winter rape of the Bn1 line while using the proposed technique. Thus, the developed system is effective and economically viable for producing of biotechnological winter rape plants.

Key words: winter rape, regeneration, organogenesis, growth regulators, vernalization, somaclonal variation

Tsitologiya i Genetika 2020, vol. 54, no. 4, pp. 73-79

  1. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, 148 Academika Zabolotnoho Str., 03143, Kyiv, Ukraine
  2. LTD Ukrainian Scientific Institute of Plant Breeding (VNIS), 30 Vasylkivska Str., 03022, Kyiv, Ukraine
  3. National University of Life and Environmental Sciences of Ukraine, 15 Heroiv Oborony Str., 03041, Kyiv, Ukraine



Hnatiuk I.S., Varchenko O.I., Kuchuk M.V., Parii M.F., Symonenko Yu.V. Development of ukrainian breeding winter rape Brassica napus L. an effective in vitro regeneration system, Tsitol Genet., 2020, vol. 54, no. 4, pp. 73-79.

In "Cytology and Genetics":
I. S. Hnatyuk, O. I. Varchenko, M. V. Kuchuk, M. F. Parii & Yu. V. Symonenko Development of an Effective In Vitro Regeneration System for Ukrainian Breeding Winter Rape Brassica napus L., Cytol Genet., 2020, vol. 54, no. 4, pp. 341346
DOI: 10.3103/S0095452720040039


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