Тhe development of tumor-induced bystander effect and radiosensitivity of glioblastoma patients' peripheral blood lymphocytes with with different status of the MGMT gene in tumor cells

Zemskova O.V., Kurinnyi D.A., Rushkovsky S.R., Demchenko O.M., Romanenko M.G, Glavatsky O.Ya, Klymenko S.V.

State Institution «Institute of Neurosurgery. acad. A.P. Romodanova National Academy of Medical Sciences of Ukraine», street Platona Maiboroda, 32, Kyiv, 04050, Ukraine
State Institution «National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine», st. Yu. Ilyenko, 53, Kyiv, 04050, Ukraine
Educational and Research Center «Institute of Biology and Medicine» of Taras Shevchenko National University of Kyiv, st. Volodymyrska, 64/13, Kyiv, 01601, Ukraine

SUMMARY. The effect of the MGMT gene methylation in glioblastoma cells on the development of the tumor-in-duced bystander effect and the modification of the sensitivity of untransformed bystander cells (peripheral blood lymphocytes) to the impact of ionizing radiation was studied. The results obtained demonstrate the development of the bystander effect, induced by the glioblastoma. There was no association found between the manifestation of the tumor-induced bystander effect in non-irradiated peripheral blood lymphocytes and the methylation status of the promoter region MGMT gene in the tumor cells. After the exposure to the radiation, statistically significant (p < 0,01) differences in apoptotic activity and radiosensitivity in peripheral blood lym-phocytes of glioblastoma patients with different status of the MGMT gene methylation in tumor cells were observed.

Keywords: methylation MGMT, tumor-induced witness effect, Comet assay, glioblastoma

TSitologiya i Genetika
2021, vol. 55, no. 2, 24-31

Cytology and Genetics
2021, vol. 55, no. 2, 125–131,
doi: 10.3103/S0095452721020158

Full text and supplemented materials

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