SUMMARY. The systematic position of the strain Bacillus sp. 20F was found to be an antagonist of phytopathogenic bacteria and micromycetes with pronounced phosphate-mobilizing properties. It was shown that the set of cultural-morphological and physiological-biochemical properties of the strain belongs to the group Bacillus subtilis. The fatty acids of the cell wall of the strain are mainly branched derivatives of iso- and anteiso-C15:0 and C17:0 fatty acids (approximately 82 %), which is characteristic of the species Bacillus amyloliquefaciens. According to the analysis of the nucleotide sequence of the 16S rRNA gene and the study of the profile of polymorphic nucleotides, the strain is classified as Bacillus velezensis.
Keywords: Bacillus sp. 20F, cultural and morphological features, physiological and biochemical properties, fatty acid composition, molecular genetic analysis, identification, systematic position
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