SUMMARY. Actinobacteria isolated from poorly explored biotopes are of interest as producers of biologically active compounds. Here, we report about the producer of lydicamycin Je 1–6, isolated from the rhizosphere soil of J. excelsa Bieb. collected from the Crimean Peninsula, Ukraine, that demonstrated its antagonistic activity against gram-positive bacteria. Based on the 16S rRNA gene sequence analysis, the strain Je 1–6 was affiliated to the Streptomyces genus. The crude extract from the biomass of this strain showed its activity against gram-positive bacteria, including polyresistant clinical isolates of Staphylococcus sp. The dereplication analysis of the extract of strain Je 1–6 resulted in determining lydicamycin and its congeners: 30-demethyllydicamycin, 30-demethyl-8-deoxylydicamycin and 8-deoxylydicamycin. MLSA analysis based on the 16S rRNA gene and five housekeeping genes atpD, gyrB, rpoB, recA, and trpB of the strain Je 1–6 showed a high level of homology with Streptomyces sp. ID38640.
Keywords: Streptomyces, MLSA, phylogenetic analysis, lydicamycin, housekeeping genes
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