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Studies on gamma rays induced cytomorphological variations and procurement of some induced novel mutants in Kalmegh [Andrographis paniculata (Burm F.) Nees.]

Dwivedi K., Kumar K., Kumar G.

 




In order to increase genetic variability for improvement and better adaptation in the present days changing climate, present investigation has been done in Andrographis paniculata (Burm. F.) Nees., (also known as Kalmegh in Ayurveda), an ethnomedicinal wild plant with highly immuneboosting properties. For the purpose, healthy, dry and homogeneous seeds were treated with four doses of gamma rays from Co60 sources at the dose rate of 1.55 Gy per second(selected on the basis of LD50i.e. 25Gy (gray), 50Gy, 100Gy and 200Gy along with one set of nonirradiated seeds and sown in triplicates in a completely randomized block design (CRBD). Higher doses were detrimental hence not suggested for further mutation breeding experiments. Ho
wever, lower doses (viz. 25Gy and 50Gy) of gamma rays were stimulatory; had induced some significant (p > 0.5) variations in several lucrative traits of Andrographis paniculata (Kalmegh) viz. Plant height, leaf area, number of branches per plant, length of internodes (mm), fruit length, and seeds per fruit. Some induced novel mutants (viz. bushy, dark green leaves mutant and tricotyledonous leaf mutant in M2 generation) have also been procured. This is, to the best of our knowledge, first report on induction of tricotyledonous leaf mutant in A. paniculata using gamma rays.

Key words: Andrographis paniculata (Kalmegh), Chromosomal aberrations, Gamma rays, Genetic variation, Meiosis, Tricotyledon, Wild medicinal plants

Tsitologiya i Genetika 2021, vol. 55, no. 4, pp. 72-73

  1. Cytogenetics, Plant Breeding, Molecular Biology, and Biotechnology laboratory, University Department of Botany, Ranchi University, India834001
  2. Naithani Plant Genetics Laboratory, Department of Botany, University of Allahabad, India211001

E-mail: kshama.dwivedi gmail.com

Dwivedi K., Kumar K., Kumar G. Studies on gamma rays induced cytomorphological variations and procurement of some induced novel mutants in Kalmegh [Andrographis paniculata (Burm F.) Nees.], Tsitol Genet., 2021, vol. 55, no. 4, pp. 72-73.

In "Cytology and Genetics":
Kshama Dwivedi, Kamini Kumar & Girjesh Kumar Studies on Gamma Rays Induced Cyto-Morphological Variations and Procurement of Some Induced Novel Mutants in Kalmegh [Andrographis paniculata (Burm. f.) Nees], Cytol Genet., 2021, vol. 55, no. 4, pp. 379387
DOI: 10.3103/S0095452721040034


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