TSitologiya i Genetika 2021, vol. 55, no. 6, 75-77
Cytology and Genetics 2021, vol. 55, no. 6, 566–575, doi: https://www.doi.org/10.3103/S0095452721060025

Enhanced in vitro regeneration in sugarcane (Saccharum officinarum L.) by use of alternate high-low picloram doses and thidiazuron supplementation

Akram M.S., Alvi A.K., Iqbal J.

  1. School of Biological Sciences, University of the Punjab (54590), Lahore, Pakistan
  2. Department of Botany, Government College University (38000) Faisalabad, Pakistan
  3. Department of Botany, Government College Women University Faisalabad, Pakistan

Sugarcane is an important domestic and industrial crop and considerable efforts have been made to improve its yield through conventional as well as biotechnological approaches. Genetic manipulation of sugarcane is dependent on an efficient reproducible in vitro regeneration regime. In the current study, the role of explant position, induced desiccation, picloram (PIC) levels for callogenesis as well as thidiazuron (TDZ) addition during regeneration phase has been appraised. Using an optimum combination of mentioned factors, an enhanced in vitro regeneration system has been established for two elite sugarcane cultivars. Embryogenesis was stimulated in cv. HSF-240 by MS medium augmented with 12.42 µM PIC while cv. CPF-237 exhibited sometic embryo formation when PIC supplementation was combined with induced desiccation (using 12 g L–1 agar). A decrease in embryogenesis frequency was recorded from base towards tip. The explants cultured alternately on high (12.42 µM) and low (4.14 µM) PIC medium produced the highest number of nodular calli which later exhibited maximum regeneration potential. Optimal shoot initiation was observed with 9.08 µM TDZ followed by medium having 2.27 µM 2,4-dichlorophenoxyacetic acid plus 4.43 µM benzylaminopurine. However, the shoots produced with former medium composition showed frailty as compared to the one regenerated on later medium. Healthy roots were initiated with 16.11 µM naphthalene acetic acid in the presence of 0.5 % activated charcoal. Malondialdehyde content, catalase and peroxidase activity of in vitro and field grown sugarcane plants were analogous, indicating that the in vitro regenerated plants were equally fit for subsequent growth in natural conditions. The reported protocol can be helpful in devising strategies for a robust sugarcane genetic engineering regime.

Keywords: Auxins, Callogenesis, Explant; Growth regu-lators, Somatic embryogenesis

TSitologiya i Genetika
2021, vol. 55, no. 6, 75-77

Current Issue
Cytology and Genetics
2021, vol. 55, no. 6, 566–575,
doi: 10.3103/S0095452721060025

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