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Study on effects of AgSiO2 core shell nanoparticles on biocompatibility appraisal of myoblasts
The main goal of present study was to check interaction of Ag-SiO2 core shell nanoparticles (CSNs) with C2C12 cells. We herein account the synthesis and classification of novel CSNs. We aimed the preparation of CSNs in order to check their biocompatible/cytotoxic impact on C2C12 muscle cells. We aimed to check the CSNs related hazards to human health. The utilized CSNs were synthesized by the straightforward sol-gel method utilizing silver nitrate and tetraethoxysilane as fundamental components. The physicochemical characterization of the CSNs was conceded by means of X-ray diffraction, UV-Vis and transmission electron microscopy. To examine in vitro biocompatibility/cytotoxicity, C2C12 cells were cultured under in vitro environment and afterward subjected to different concentrations of CSNs. The survival of C2C12 cells was evaluated via cell counting Kit-8 assay at exact point gap. These results were authenticated by confocal microscopy. The morphology of C2C12 cells was observed with the help of a phase contrast microscope. In vitro investigation of biological effects of CSNs has revealed that feasibility of cells in culture follows dose (0–20 µg/ml) mode. Overall, results of this investigation demonstrate that synthetic CSNs influence C2C12 cell practicability with time point as well as absorption dependent mode.
Key words: Core shell; Biomedical; Nanoparticles, C2C12, Silica
E-mail: touseefamna gmail.com, m.skhil jbnu.ac.kr
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