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Genetic diversity of potato virus Y in Belarus
SUMMARY. The strain composition of PVY from Belarus as well as the molecular variability of their genomes were studied. The presence of the strains PVYNTN (genotype A) and PVYNW (genotypes A and B) was shown using RT-PCR. The degree of identity between individual genome regions of isolates studied varies from 100 to 97.33 %. Nucleotides that are under purifying selection revealed in sequences. The study of the phylogenetic relationships of viral isolates from Belarus and other countries showed that there was no clear correlation between geographical origin or host plant species and the viral genomes fragments similarity degree. The high similarity of Y-virus genomes fragments obtained from different sources indicates that studied loci are highly conservative, which allows to use the molecular markers for accurate and quick viral infections detection and virus-free plants revealing. The studied fragments Y-virus genomes obtained from different sources were highly similar that indicates the conservatism of these loci. Therefore, molecular markers to these fragments can be used to accurately and quickly viral infections detection as well as virus-free plants revealing.
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