TSitologiya i Genetika 2021, vol. 55, no. 3, 3-9
Cytology and Genetics 2021, vol. 55, no. 3, 207–212, doi: https://www.doi.org/10.3103/S0095452721030051

Temperature stress response of althaea officinalis «hairy» root lines carrying human interferon α2b gene

Matvieieva N.A., Ratushnyak Y.I., Duplij V.P., Shakhovsky A.M., Kuchuk M.V.

  • Institute of Cell Biology and Genetic Engineering of National Academy of Sciences of Ukraine, 148 Academika Zabolotnoho St., 03143, Kyiv, Ukraine

SUMMARY. «Hairy» roots, obtained by genetic transformation of plants using soil phytopathogenic bacteria Agrobacterium rhizogenes, are valuable producers of important se-condary metabolites with medicinal properties and a
convenient model object for studying the response of plants to adverse environmental conditions. This paper compares the postponed response of «hairy» roots of Althaea officinalis L. to the short-term cold and high-temperature stresses. The results indicate that the «hairy» roots of different lines of A. officinalis (individual transformational events) differ in sensitivity to short-term temperature stress, regardless of the vector used for the transformation and the presence of human interferon ifn-α2b gene. The high temperature led to significant inhibition of root growth of all lines, except the one that had the highest content of flavonoids under the control conditions. On the other hand, short-term cultivation of «hairy» roots at low temperature did not cause growth inhibition. Simultaneously with the inhibition of growth by high temperature conditions, an increase in the synthesis of flavonoids was observed. Probably, it was a response of the roots to the action of high temperature as a stress factor. The strong (R2 = 0,78) linear dependence between antioxidant activity of «hairy» root extracts and the total flavonoid content was determined. Thus, flavonoids synthesized in A. officinalis «hairy» roots may be involved in the process of response and adaptation of the roots to high temperature stress.

Keywords: Agrobacterium rhizogenes, Althaea officinalis, “hair” roots, temperature stress, flavonoids, antioxidant activity

TSitologiya i Genetika
2021, vol. 55, no. 3, 3-9

Current Issue
Cytology and Genetics
2021, vol. 55, no. 3, 207–212,
doi: 10.3103/S0095452721030051

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