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Temperature stress response of althaea officinalis hairy root lines carrying human interferon α2b gene

Matvieieva N.A., Ratushnyak Y.I., Duplij V.P., Shakhovsky A.M., Kuchuk M.V.


SUMMARY. Hairy roots, obtained by genetic transformation of plants using soil phytopathogenic bacteria Agrobacterium rhizogenes, are valuable producers of important se-condary metabolites with medicinal properties and a
convenient model object for studying the response of plants to adverse environmental conditions. This paper compares the postponed response of hairy roots of Althaea officinalis L. to the short-term cold and high-temperature stresses. The results indicate that the hairy roots of different lines of A. officinalis (individual transformational events) differ in sensitivity to short-term temperature stress, regardless of the vector used for the transformation and the presence of human interferon ifn-α2b gene. The high temperature led to significant inhibition of root growth of all lines, except the one that had the highest content of flavonoids under the control conditions. On the other hand, short-term cultivation of hairy roots at low temperature did not cause growth inhibition. Simultaneously with the inhibition of growth by high temperature conditions, an increase in the synthesis of flavonoids was observed. Probably, it was a response of the roots to the action of high temperature as a stress factor. The strong (R2 = 0,78) linear dependence between antioxidant activity of hairy root extracts and the total flavonoid content was determined. Thus, flavonoids synthesized in A. officinalis hairy roots may be involved in the process of response and adaptation of the roots to high temperature stress.

Key words: Agrobacterium rhizogenes, Althaea officinalis, hair roots, temperature stress, flavonoids, antioxidant activity

Tsitologiya i Genetika 2021, vol. 55, no. 3, pp. 3-9

  • Institute of Cell Biology and Genetic Engineering of National Academy of Sciences of Ukraine, 148 Academika Zabolotnoho St., 03143, Kyiv, Ukraine

E-mail: duplijv

Matvieieva N.A., Ratushnyak Y.I., Duplij V.P., Shakhovsky A.M., Kuchuk M.V. Temperature stress response of althaea officinalis hairy root lines carrying human interferon α2b gene, Tsitol Genet., 2021, vol. 55, no. 3, pp. 3-9.

In "Cytology and Genetics":
N. A. Matvieieva, Y. I. Ratushnyak, V. P. Duplij, A. M. Shakhovsky & M. V. Kuchuk Effect of Temperature Stress on the Althaea officinaliss Hairy Roots Carrying the Human Interferon α2b Gene, Cytol Genet., 2021, vol. 55, no. 3, pp. 207212
DOI: 10.3103/S0095452721030051


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