Цитологія і генетика 2021, том 55, № 1, 98-100
Cytology and Genetics 2021, том 55, № 1, 96–104, doi: https://www.doi.org/10.3103/S0095452721010059

Genetic Variability in Winter Rye (Secale cereale L.) Accessions at Early Stage of Self-pollination Manifested through Fertility, Plant height and Secalins

Daskalova N., Doneva S., Spetsov P.

  1. Plant Production Department, Technical University, Varna, 9010 Bulgaria
  2. Dobrudzha Agricultural Institute, General Toshevo, 9520 Bulgaria
  3. Aksakovo Center, Aksakovo, Varna region, 9154 Bulgaria

РЕЗЮМЕ. Селекцію озимих самозапильних рослин з невеликою або середньою висотою стебла ініціювали у 15 популяціях культурного жита. Протягом перших двох років було зафіксовано близько 8,8% насіння на одному самозапиленому колоску. У інбредних сімействах S2 спостерігали самозапилення у діапазоні від 0 до 73 зернин на окремий колос, і висота рослин складала від 76 до 115 см. Реакція на селекцію та генетичне удосконалення у процентному вираженні середнього числа характеризувало сімейства S3 щодо одного циклу селекції. Зразки ДНС-ПААГ продемонстрували дві основні смуги протеїну для високомолекулярних секалінів жита – одна х-субодиниця з повільним рухом, виражена як 2r, 5,2*r і 5,3r, і друга у-субодиниця з швидким рухом, виражена як 6r, 6,5r, 7r і 9r. Що стосується алелів у локусах Glu-R1 та Gli-R2, 9 нащадків продемонстрували генетичну гомогенність у білках, що супроводжувалося низькими коефіцієнтами зміни висоти рослини. Високомолекулярні комбінації 2r (окрема смуга) і 5,3r+7r були визначені як нові субодиниці секаліну. 75K γ-секаліни, кодовані у Gli-R2, складалися з алелів a, b, c для субодиниць d1, d2 і t1, відповідно. Результати продемонстрували, що чотири, з-поміж усіх, нащадки самозапиленого жита є гомогенними генотипами, і їх можна використовувати як інбредні лінії у подальших генетичних та селекційних експериментах.

Ключові слова: жито, інбредні лінії, висота рослин, дата колосіння, самозапилення, секаліни, Glu-R1, Gli-R2

Цитологія і генетика
2021, том 55, № 1, 98-100

Current Issue
Cytology and Genetics
2021, том 55, № 1, 96–104,
doi: 10.3103/S0095452721010059

Повний текст та додаткові матеріали

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