Characterization and phylogeny analysis of Azerbaijan tea (Camellia sinensis L.) genotypes by molecular markers

Huseynov M., Suleymanova Z., Ojaghi J., Mammadov A.

Lankaran State University, Ministry of Education of Azerbaijan, Lankaran, 50 H. Aslanov
Institute Molecular Biology and Biotechnologies of National Academy of Sciences of Azerbaijan, AZ1073, Baku, 11 Izzat Nabiyev
Center for Cell Pathology Research, Department of Life Sciences, Khazar University, Azerbaijan

РЕЗЮМЕ. Чай – один з найважливіших продуктів у південному регіоні Республіки Азербайджан, який відіграє значну роль в економіці регіону. Оцінка та опис генетичного різноманіття рослин цього виду є значимим першим кроком до їхнього вдосконалення. У цьому дослідженні генотипи чаю з південного регіону Азербайджану досліджували за використання 10 випадково ампліфікованих поліморфних ДНК для того, щоб оцінити їхнє гене-тичне різноманіття і встановити відносини між їхніми генотипами. Праймери RAPD згенерували 132 продуктів ампліфікації і 113 з них були поліморфними. Поліморфні паттерни смугастості з певною кількістю ампліфікованих фрагментів були у діапазоні від 4 (OPA-19) до 19 (OPAB-18). Діапазон відсотків поліморфізму складав від 50 до 95 % з середнім значенням в 83,94 %. Генетична подібність між генотипами, які тестували, перебувала у діапазоні від 0,445 до 0,819 при середньому значенні в 0,512. Кластерний аналіз на основі UPGMA та індексу подібності Жаккара виявили 5 основних кластерів для даних RAPD, а аналіз головних координат (PCoA) підтвердив результат кластерингу. Згідно з нашими результатами, між генотипами чаю на півдні Республіки Азербайджан існує відносно велика генетична відстань. Крім того, можна зробити висновок, що маркери RAPD – це прийнятні інструменти для оцінки генетичного різноманіття і відносин у межах Camellia sinensis.

Ключові слова: Camellia sinensis, маркер RAPD, генетична мутація, PCoA, поліморфізм

Цитологія і генетика
2022, том 56, № 3, 73-75

Cytology and Genetics
2022, том 56, № 3, 285–291,
doi: 10.3103/S0095452722030057

Повний текст та додаткові матеріали

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