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The Investigation of Genomes of Some Gentiana Species in the Nature and in vitro Cell Culture

Mel'nik V.M., Spiridonova K.V., Andreev I.O., Strashnyuk N.M., Kunakh V.A.

 




The comparative study of the genomes of intact plants-representatives of some species of the genus Gentiana L. as well as cultured cells of G. lutea and G. punctata was performed using restriction analysis. Species specificity of restriction fragment patterns for studied representatives of this genus was revealed. The differences between electrophoretic patterns of digested DNA purified from rhizome and leaves of G. lutea and G. punctata were found. The changes in genomes of G. lutea and G. punctata cells cultured in vitro compared with the genomes of intact plants were detected. The data obtained evidence that some of them may be of nonrandom character.

Tsitologiya i Genetika 2002, vol. 36, no. 6, pp. 28-34



Mel'nik V.M., Spiridonova K.V., Andreev I.O., Strashnyuk N.M., Kunakh V.A. The Investigation of Genomes of Some Gentiana Species in the Nature and in vitro Cell Culture, Tsitol Genet., 2002, vol. 36, no. 6, pp. 28-34.




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