Stimulation of cholinogenesis in the human fetal nerve cells culture

Tsymbaluk V.I., Vasilyeva I.G., Olexenko N.P., Chopic N.G., Tsybko O.I., Galanta E.S.

SUMMARY. The aim of the research was to establish cultured population of nerve cells reached by cholinergic neurons and their determinative precursors. The most effective combination of neuroinductors which stimulated cholinergic cells differentiation from the nerve stem cells was retinoic acid and acetylcholine. During the period of culturing the amount of ChAT⁺ cells reliably increased from 5,3 ± 2,9% to 21,1 ± 6,2%. At the same time in the control samples their concentration was 9,1 ± 4,8% of total cell count. Enrichment of cell population by cholinergic neurons and their determinative precursors correlated with increasing of AChE-activity level. So, addition of retinoic acid and acetylcholine stimulate both neurogenesis and cholinogenesis in the culture of human fetal nerve cells.

Key words: Stem cells, ChAT, AChE, fetal nerve cells culture, acetylcholine, retinoic asid

Tsitologiya i Genetika 2013, vol. 47, no. 3, pp. 54-59

E-mail: N.Oleksenko gmail.com

Tsymbaluk V.I., Vasilyeva I.G., Olexenko N.P., Chopic N.G., Tsybko O.I., Galanta E.S. Stimulation of cholinogenesis in the human fetal nerve cells culture, Tsitol Genet., 2013, vol. 47, no. 3, pp. 54-59.

In "Cytology and Genetics":
V. I. Tsymbalyuk, I. G. Vasil’eva, N. P. Oleksenko, N. G. Chopik, O. I. Tsyubko, O. S. Galanta Stimulation of cholinogenesis in human fetal nerve cell cultures, Cytol Genet., 2013, vol. 47, no. 3, pp. 174-178
DOI: 10.3103/S0095452713030109