The role of oxidative stress in apoptosis and cell proliferation of human bronchial epithelial cells

Ecevit H., Urhan-Kucuk M., Uluca H., Tap D., Arpaci A.

Oxidative stress is an important pathophysiological factor in chronic respiratory diseases. Our study aimed at elucidating through which pathway oxidative stress–mediated apoptosis occurs at the gene expression level under oxidative stress in the human bronchial epithelial cell line BEAS-2B. Suitable doses and time period were detected by exposing BEAS-2B cells to hydrogen peroxide (H₂O₂) at different doses and time periods, and the oxidative-damaged cell culture model was designed. The treatment and control groups were compared in terms of gene expression levels determined by Quantitative Real Time Polymerase Chain Reaction. The oxidative-damaged cell model was confirmed by the spectrophotometric measurement of malondialdehyde and catalase activity (p < 0.05). Caspase-3, caspase-9, bax, and bak gene expression levels increased significantly in the treatment group compared to the control group (p < 0.05). There were not any significant differences between the groups in terms of caspase-8, Bcl-2, and bik (p > 0.05). p53 and p21 gene expression levels were found to be significantly higher in the treatment groups (p < 0.05). H₂O₂-induced oxidative stress, induced apoptosis through the intrinsic pathway at gene expression level in the bronchial epithelial BEAS-2B cells was observed.

Key words: Apoptosis, BEAS-2B, Cell proliferation, Oxidative stress, Reactive oxygen species

Tsitologiya i Genetika 2021, vol. 55, no. 3, pp. 80-81

1. Hatay Mustafa Kemal University, Medical Faculty, Department of Medical Biology, Hatay, Turkey
2. Hatay Mustafa Kemal University, Health Sciences Institute, Department of Molecular Biochemistry and Genetics, Hatay, Turkey
3. Hatay Mustafa Kemal University, Medical Faculty, Department of Biochemistry, Hatay, Turkey

E-mail: meralurhan hotmail.com, hasretecevit gmail.com, ulucahaluk gmail.com, duygu.tap hotmail.com, arpaci57 gmail.com

Ecevit H., Urhan-Kucuk M., Uluca H., Tap D., Arpaci A. The role of oxidative stress in apoptosis and cell proliferation of human bronchial epithelial cells, Tsitol Genet., 2021, vol. 55, no. 3, pp. 80-81.

In "Cytology and Genetics":
Hasret Ecevit, Meral Urhan-Kucuk, Haluk Uluca, Duygu Tap & Abdullah Arpaci The Role of Oxidative Stress in Apoptosis and Cell Proliferation of Human Bronchial Epithelial Cells, Cytol Genet., 2021, vol. 55, no. 3, pp. 283–289
DOI: 10.3103/S0095452721030026

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