Огляд присвячено аналізу сучасних досягнень метаболічної інженерії Corynebacterium glutamicum для продукування лізину. Розглянуто ключові гени біосинтезу лізину у C. glutamicum та шляхи створення нових генетично модифікованих штамів. Описана роль різних плазмід, конструювання векторних касет та види промоторів для регуляції експресії генів у C. glutamicum. Наведено інформацію з використання вуглецевмісних субстратів (гексоз, пентоз, молочної кислоти, манітолу) для виробництва лізину. Розглянуто можливості використання технології CRISPR в генетичній інженерії C. glutamicum. Генетичні зміни C. glutamicum дозволили використання альтернативних субстратів та сприяли підвищенню рівня накопичення лізину в культуральній рідині. Узагальнено дані, які можуть слугувати для створення нових штамів-надпродуцентів лізину.
РЕЗЮМЕ. Обзор посвящен анализу современных достижений метаболической инженерии Corynebacterium glutamicum для накопления лизина. Рассмотрены ключевые гены биосинтеза лизина в C. glutamicum и пути создания новых генетически модифицированных штаммов. Описана роль различных плазмид, конструирование векторных кассет и виды промоторов для регуляции экспрессии генов в C. glutamicum. Приведена информация по использованию углеродсодержащих субстратов (гексоз, пентоз, молочной кислоты, маннитола) для производства лизина. Рассмотрены возможности использования технологии CRISPR в генетической инженерии C. glutamicum. Генетические изменения C. glutamicum позволили использование альтернативных субстратов и способ-ствовали повышению уровня накопления лизина в культуральной жидкости. Обобщены данные, которые могут служить для создания новых штаммов-суперпродуцентов лизина.
Ключові слова: Corynebacterium glutamicum, штам-продуцент, мікробіологічний синтез, лізин, метаболічна інженерія
Повний текст та додаткові матеріали
Цитована література
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